Carlos José Botelho Junior, Brazilian physician (São Paulo c.1884 – São Paulo 12 April 1960)

Observed that citric acid enables iodine in potassium iodide to precipitate proteins and sera more readily in the serum of cancer patients that in that of normal subjects (1916) and developed Botelho reaction or test based on it that was improved by Ichikawa (1928)

Presented Botelho reaction at Academie des Sciences de Paris (1917) 

Improved the Botelho reaction employing refractometry

Proposed a formula to use in Botelho’s reaction (Q=40/i, being Q the desired amount of serum and i the refractometric index of serum in albumin equivalents)   

Created Hartmann-Botelho solution for local treatment of ulcerative cancer with H. Hartmann in Presse Medicale 32 (1918)

With H. Hartmann. Resultats experimentaux de tentatives d’inocculation de cancer humain au chien

First to graft human tumors into animals (horses)

Quelques recherches experimentales sur le cancer. Compt. Rend. Soc. Biol. 81:1114, 1918

Mudança de cor de uma solução iodo-iodina quando em presença de órgãos considerados como tendo um poder anti-blastico ou pro-blastico (1937)

DESCRIBED METHODS

Sur un nouvelle methode pour la mise en evidence de bleu de methylene dans le urines. Comptes Rendus Seances Soc. Biol. 81:291, 1918

Sur une nouvelle methode simple et rapide pour la double coloration des bactéries sporulées (A description of a new and simple method for double staining of sporulated bacteria). Comptes Rendus Seances Soc. Biol. 81:183-4, 1918 

Milieu pour déceler les bacilles du groupe typhique dans un milieu impur (Culture medium for rapidly detecting the presence of bacilli of the typhoid group). Comptes Rendus Seances Soc. Biol. 80:435-7, 1917 & J.R. Micro. Soc. 425, 1917

Sur un nouveau milieu de culture indiquant rapidement la presence de bacilles du groupe typhique dans un milieu bacteriologiquement impur. (A description of a new culture media for the rapid detection of the presence of bacilli of the typhus group in bacteriologically impure media). Compt. Rend. Soc. Biol. 80:435-7, 1917 

Notas complementares a propósito da técnica da biocromoreação. Ann. Paul. Med. Cir. 2(2):64, 1914

Technique of bichromo-reaction applied to the bacteriologic diagnosis of typhoid fever. Compt. Rend. Soc. Biol. 74:118-20, 1913

Biocromoreação: novo método de pesquisa do bacilo de Eberth nas fezes de tíficos com aplicação ao diagnóstico bacteriológico precoce da febre tifóide. Ann. Paul. Med. Cir. 1(5):137-49, 1913

A new method for the immediate demonstration of the bacillus of Eberth in typhoid faecal matter, applied to the early bacteriologic diagnosis of typhoid fever; the bichromo-reaction. Compt. rend. Soc. Biol. 73:692, 1912

DESCRIBED TRYPANOSOMES

Trypanosoma pedrozi 1914 with Carini

Sur deux nouveaux trypanosomes des poissons. C.R. Soc. Biol. Paris 59(2):128-9, 1907
(Trypanosoma rhamdiae 1907)