Americo Pomales Lebron
Americo Pomales Lebrón, Puerto Rican microbiologist (Guayama 01 January 1904 – 16 December 1984) DEVELOPED METHODS With B.M. Thomason, W.B. Cherry & B.R. Davis. Rapid presumptive identification of enteropathogenic Escherichia coli in faecal smears by means of fluorescent antibody. I. Preparation and testing of reagents. Bull. World Health Org. (1961) With C. Fernandez. A method for estimating the number of bacteria in liquids and tissues. J. Bacteriol. 64(6):837-45, 1952 With P. Morales Otero. A method for preservation of oxalate plasma for fibrinolytic tests. Proc. Soc. Exp. Biol. Med. 41:609, 1939
Amanda Pellegrino de Iraldi
Amanda Angela Pellegrino de Iraldi, Argentine (San Nicolás 1917 – Buenos Aires 22 February 2007) Daughter of Italian immigrants Authored over 150 works ACHIEVEMENTS First to describe spontaneous atherosclerosis of female rat Obtained the first experimental generalized atherosclerosis First to identify adrenergic synaptic vesicles First to formulate the coexistence of neurotransmitters in nervous system METHODS With G. Rodríguez de Lores & E. De Robertis developed original procedures for isolating synaptosomes and synaptic vesicles With G. Rodríguez de Lores & E. De Robertis developed De Robertis method of differential centrifugation for isolation of synaptic terminals (1962) With C.J. Tandler. A silver-reducing component in rat striated muscle. I. Selective localization at the level of the terminal cistern/transverse tubule system. Light and electron microscope studies with a new histochemical procedure. Histochemistry 92(1):15-22, 1989
Aluizio Prata
Aluízio da Rosa Prata, Brazilian infectologist and parasitologist (Uberaba, Minas Gerais State 01 June 1920 – Uberaba, Minas Gerais State 13 May 2011) Authored over 275 papers, 48 book chapters and 7 books Considered as a world expert on schistosomiasis Showed foundations and applications of retal biopsy in schistosomiasis diagnosis and evaluation of its therapy. Beyond this developed a method for finding Schistosoma in animal organs A biópsia retal na esquistossomose mansoni e tratamento. Doctoral Thesis, Faculdade de Medicina da Universidade Federal da Bahia (1957) Described an advanced hepatic form of schistosomiasis without splenomegaly (with Z. Andrade) Fibrose hepática de Symmers sem esplenomegalia. O Hospital 63:617-23, 1963 With Bina first demonstrated clinically the degradation and removal of a long standing hepatic fibrosis in a human disease, the hepatosplenic schistosomiasis (1983) MEMBERSHIP Visiting Professor, Cornell University (1965) Honorary Fellow, Royal Society of Tropical Medicine and Hygiene (1991) Honorary Member, American Society of Tropical Medicine and Hygiene (1998)
Allan Robert Phillips
Allan Robert Phillips, US-born Mexican ornithologist (New York City 25 October 1914 – San Nicolás de los Garza, Nuevo León State 26 January 1996) DESCRIBED AVIAN TAXA Valid Species Empidonax oberholseri 1939 Leucolia wagneri 1966 Phaethornis griseoventer 1962 Subspecies 154
Alfred Polson
Alfred Gustave Polson, South African biochemist (Brandfort, OFS 11 March 1912 – 2001) Demonstrated the possibility of protein fractioning of plasma by polyethylene glycol precipitation (1964) Developed a number of techniques for separation and purification of animal and plant viruses such as precipitation by polyethylene glycol New amino acids in bacterial hydrolysates. Nature, 1948 METHODS/TECHNIQUES A new method for measuring diffusion constants of biologically active substances. Nature 154:223, 1944 A quantitative chromatographic method of analysis on filter paper (1948) With J. Dent & S.M. Lawrence. Improved diluents for the production of African horse-sickness virus vaccine. Nature, 1951 With G. Selzer. A rapid method for the purification of the MEF 1 strain of poliomyelitis virus. Biochim. Biophys. Acta 14:67, 1954 A method for determination of diffusion coefficients by the gel precipitin techniques. Biochim. Biophys. Acta 29:426, 1958 A new method for measuring diffusion constants (1960) With M.H.V. Van Regenmortel. A new method for determination of sedimentation constants of viruses. Virology 15(4):397-403, 1961 A simple procedure for forming density gradients (1963) With B. Russel & T.H. Mead. A method of preparing agarose. Biochim. Biophys. Acta 86, 1964 With M.B. Von Wechmar. A new way to transmit plant viruses. J. Gen. Virology 51:179-81, 1980 With H. Alk & K. Achleitner. The sedimenting-droplet procedure for measuring fluid densities. Anal. Biochem. 107(1):25-31, 1980 With H. Epstein & K.J. Van der Merwe. A novel method of electrophoresis for the separation of individual human serum glycoproteins. Prep. Biochem. 17(2):109-20, 1987 Electro-extraction method and apparatus for rapid extraction of viruses from infected plant tissues (1993) DEVICES An electrophoresis apparatus for the rapid routine analysis of sera and other protein solutions. Ondersteport J. Vet Research 20:159, 1944 Electrophoresis apparatus for analyzing several sera simultaneously. Nature, 1951 Improved electrophoresis cell for the analysis of several sera simultaneously. Nature, 1952 A electrodecantation apparatus for isolation and purification of protein and viruses (1953) A simplified fraction collector for gradient elution chromatography. J. Chromat. A 5:116-20, 1961 With L. Stannard a centrifugal rotor for concentrating viruses (1970) A new capillary cell for measuring the rate of sedimentation of virus particles in a centrifugal field PATENTS Method and apparatus for electro-decantation and its application for the separation of materials (1958) Fractionation of mixtures of agarose and agaropectin (1965) Chemically hardened gelatin-separating medium for chromatography and ion exchange and method for making the same (1972) with W. Katz Ultracentrifuge rotor (1974) with K.J. Kaufmann Influenza virus vaccine (1976) Recovering purified antibodies from egg yolk (1982) Carrier bound immunosorbent (1983) with K. J. Van der Merwe Carrier bound immunogenic determinants (1983) with K. J. Van der Merwe Manufacture and use of fowl egg antibodies (1985) Carrier bound immunogenic determinants and carrier therefor (1990) with K. J. Van der Merwe
Alexandrino Pedroso
Alexandrino de Moraes Pedroso, Brazilian bacteriologist (Cotia, São Paulo State 1881 – São Paulo 1922) With E. Brumpt first reported the importance of pets in epidemiological cycle of American cutaneous leishmaniasis (1913) With José Maria Gomes observed first chromoblastomycosis or Lane-Pedroso-Gomes disease (1911) but didn’t publish until 1920, losing his priority to Max Rudolph. Its etiologic agent was named Fonsecaea pedrosoi in his honor With J.M.Gomes. Sobre quatro casos de dermatite verrucosa produzida por Phialophora verrucosa. An. Paul.Med. Cir. 11:53-1, 1920 With Pedro Dias da Silva first to growing Leishmania brasiliensis in culture Introduced improvements in Nicolle medium