José Moura Gonçalves, Brazilian biochemist (Rio de Janeiro 05 January 1914 – São Paulo 19 October 1996)

ACHIEVEMENTS

With C. R. Diniz isolated and characterized the active compounds of venom from Tityus serrulatus such as tityustoxin  

Isolated neurotoxin protein crotamine  from venom of rattlesnakes and studied its pharmacological properties

With L.G. Vieira. Estudos sobre venenos de serpentes brasileiras. I. Análise eletroforética. An. Acad. Bras. Cien 22:141, 1950

Estudos sobre venenos de serpentes brasileiras.II. Crotalus terrificus crotaminicus, nova subespécie biológica. An. Acad. Bras. Cien. 28:365-7, 1956

Discovered dehydropeptidases

With V.E. Price & J. P. Greenstein. Enzymatic hydrolysis of acetyldehydroalanine. J. Biol. Chem. 169(1):225, 1947 & J. Natl. Cancer Inst. 7(6):443-6, 1947

Determination of the coefficient of absorption of light in the cells. Rev. Bras. Biol. 4(1):125-30, 1944

Sobre a determinação dos coeficientes de absorção e difusão da luz nas células. Thesis, Faculdade de Medicinas do Rio de Janeiro (1942) 

Relevant Papers

Chromatographic and immunochemical studies on lysozyme. Arch. Biochem. Biophys. 60:171, 1956

With V.E. Price & J.P. Greenstein. Ammonia nitrogen produced from isomeric peptides in kidney homogenate digests. Science 106 (2755):369-70, 1947

Methods

Developed new methods for protein isolation and characterization

Developed techniques of osmotic pressure, dilatometry and enzymes microtitration in tissue cuts

With C.R. Diniz. Separation of biologically active components from scorpion venoms by zone electrophoresis. Biochim. Biophys. 41:470, 1960