Lila Rosa Castellanos Serra, Cuban biotechnologist (18 September 1948 –

Developed new original methods for the detection of proteins, nucleic acids and lipopolysaccharides on electrophoresis gels

The methods developed in her laboratory on gel-staining technology are frequently cited in the international literature, and are now routinely employed by international scientific community

Introduced the use of imidazole in combination with zinc salts for detection of proteins, nucleic acids, lipopolysaccharides and lipooligosaccharides

CONTRIBUTIONS

With Y. Ramos, V. Besadá, L.J. Gonzalez, Ya García et al. Double fractionation by polyacrylamide gel electrophoresis (DF-PAGE): new method for quantitative proteomics studies. Biotecn. Aplic. 34:4501-3, 2016

With Y. Ramos, Y. García, Y. Perez Riverol et al. Peptide fractionation by acid pH SDS-free electrophoresis. Electrophoresis 32(11):1323-6, 2011

With E. Hardy. Negative detection of biomolecules separated in polyacrylamide electrophoresis gels. Nature Protocols 1(3):1551, 2006

With Y. Ramos & V. Huerta. An in-gel digestion procedure that facilitates the identification of highly hydrophobic proteins by electrospray ionization-mass spectrometry analysis. Proteomics 5:2729-38, 2005

With L.J. Gonzalez, V. Badock, M. Díaz, A. Moro et al. Identification of nuclear proteins of small cell lung cancer cell line H82: an improved procedure for the analysis of silver-stained proteins. Electrophoresis 24 (2003)

With A. Vallin, W. Proenza, J.P. LeCaer & J. Rossier. An optimized procedure for detection of proteins on carrier ampholyte isoelectric focusing and immobilized pH gradient gels with imidazole and zinc salts. Its application to the identification of isoelectric focusing separated isoforms by in-gel proteolysis and mass spectrometry. Electrophoresis (2001)

With W. Proenza, V. Huerta & R.J. Simpson. Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts. Electrophoresis 20:7 32-7, 1999

With C. Fernandez-Patrón, E. Hardy Rando, M. Guerra, E. Estevez, E. Mehl & R.W. Frank. Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: generalization of the reverse-staining technique. Electrophoresis (1998)

With E. Hardy, E. Pupo, H. Santana & M. Guerra. Elution of lipopolysaccharides from polyacrylamide gels. Anal. Biochem. (1998)

With C. Fernandez-Patrón, E. Hardy, H. Santana & V. Huerta. High yield elution of proteins from sodium dodecyl sulfate-polyacrylamide gels at the low picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins. J. Protein Chem. (1997)

With C. Fernandez-Patrón, E. Hardy & V. Huerta. A procedure for protein elution from reverse stained polyacrylamide gels applicable at the low picomole level: an alternative route to the preparation of low abundant proteins for microanalysis. Electrophoresis 17:1564-72, 1996

With C. Fernandez-Patrón, J. Madrazo, E. Hardy, E. Mendez & R.W. Frank. Single-step electrotransfer of reverse stained proteins from sodium dodecyl sulfate polyacrylamide gel onto reversed-phase minicartridge and subsequent desalting and elution with a conventional high-performance liquid chromatography gradient system for analysis. Electrophoresis (1995)

With C. Fernandez-Patrón & P. Rodríguez. Reverse staining of sodium dodecylsulfate polyacrylamide gels by imidazole-zinc salts: sensitive detection of unmodified proteins. BioTechniques 12:564-73, 1992