Roberto Eusebio Mancini Doria, Argentine morphologist (Buenos Aires 26 September 1914 – 03 February 1977)

Son of Italian immigrants

Internationally recognized as one of world’s foremost experts on histophysiology of human testis 

Performed studies about testes functions, connective tissue in human and animals and use of fluorescent substances 

Characterized cytological, embryological and histochemically the child human testis

With R. Narvaitz & J.C. Lavieri. Origin and development of the germinative epithelium and Sertoli cells in the human testis. Cytological, cytochemical and quantitative study. Anat. Rec. 136:477-89, 1960

With O. Vilar, I.E. Lavieri, I.A. Andrade & I.I. Heinrich. Development of Leydig cells in the normal human testis. Am. J. Anat. 112:203-14, 1963

Characterized the cytology of cryptorchid testis

With E. Rosemberg, M. Cullen, J.C. Lavieri, O. Vilar, C. Bergada & J.A. Andrada. Cryptorchid and scrotal human testis. I. Cytological, cytochemical and quantitative studies. Clin. Endocr. 25:911, 1965

Introduced protein fluorescent labeling for localization and flow

With O. Vilar, J.M. Dellacha et al. Extravascular localization of fluorescent albumin, globulin and fibrinogen in connective tissue structures. J. Histochem. Cytochem. (1962)

Proposed the role of connective tissue as a transient depot for the extravascular pool of serum proteins 

With L. Lustig & B. Denduchis authored the first report on the chemical properties of the basement membrane of the seminiferous tubular wall (1975) and demonstrated a partial immunological identity among basement membranes of distinct species (1977)

With Carlos Yantorno introduced immunology into reproduction studies (1977)

METHODS/TECHNIQUES

With E. Sacerdote de Lustig introduced modifications to techniques for measuring the presence of glycogen in embryonary tissues  (1941-2)

Nueva reacción del yodo para la investigación del glucogenio en tejidos. Soc. Anat. Norm. Patol. 6:628, 1946

With R. Celani Barry. Nuevo metodo para investigar glucogenio em células de frottis. Rev. Soc. Arg. Biol. 28:6, 1942

With M.A. Etcheverry. A new reaction for polysaccharides. J. Nat. Cancer Inst. (1952)

Villamil-Mancini method for c cytochrome oxidase

SOME BIBLIOGRAPHY

With I.C. Calamera, M.C. Morgenfeld & O. Vilar. Biochemical changes of the human serum produced by chlorambricil, testosterone propionate and human chorionic gonadotropin administration. Andrologia 11:43-50, 1979 

With A.B. Mazzolli, O.D. Bustuoabad & C. Barrera. A new model for antisperm autoimmunity in guinea pigs. Int. J. Fertil. 21:49-54, 1976

With L. Lustig, B. Denduchis & N. Gonzalez. Immuno-histochemical study of rat seminiferous tubule wall structures. Int. J. Fertil. (1976) 

Immunologic aspects of testicular function (1976)

With L. Martini. Male fertility and sterility (1974)

With B. Denduchis & N. Gonzalez. Concentration of hydroxyproline in testes of hypohysectomized patients before and after treatment with gonadotropins and testosterone. J. Reprod. Fertil. 31:111-4, 1972

With O. Vilar, P. Donini & A. Perez Lloret. Effect of human urinary FSH and LH on the recovery of spermatogenesis in hypophysectomized patients. J. Clin. Endocrin. 33:388-91, 1971 

With B. Denduchis & N. Gonzalez. Collagen content of testis from hypophysectomized patients before and after treatment with gonadotropins. Acta Europ. Fertil. (1970)

With A.B. Castro & A.C. Seiguer. Electron microscopy study of the localization of labeled gonadotropins in the Sertoli and Leydig cells  of the rat testis. Proc. Soc. Exp. Biol. Med. 133:582, 1970

Effect of different types of gonadotropins on the induction and restoration of spermatogenesis in the human testis. Acta Europ. Fertil. 1:401-29, 1969

With A.C. Seiguer & A.P. Lloret. Effect of gonadotropins on the recovery of spermatogenesis in hypophysectomized patients. Clin. Endocr. Metab. 29:467-78, 1969

With L. Lustig & H. Costantini. Serological and immunofluorescence study of soluble collagen in tissue culture of fibroblasts (1969)

Testiculo humano (1968)

With A. Castro & A.C. Seiguer. Histologic localization of follicle-stimulating and luteinizing hormones in the rat testis. J. Histochem. Cytochem. 15:516-25, 1967

With J.C. Lavieri, F. Muller, J.A. Andrada & D.J. Saraceni.  Effect of prednisolone upon normal and pathologic human spermatogenesis. Fertil Steril. 17:500-13, 1966

With J.A. Andrada, C. Saraceni, A.E. Bachmann, J.C. Lavieri & M. Nemirovsky. Immunological and testicular response in men sensitized with human testicular homogenate. J. Clin. Endocr. Metabol. 25, 1965

With J. Alonso, J. Barquet, B. Alvarez & M. Nemirovsky. Histoimmmunological localization of hyaluronidase in bull testis. J. Reprod. Fertil. 8:325-30, 1964

With O.V. Davidson, O. Vilar, M. Nemirovsky & D.C. Bueno. Acrosomalantigenicity in rat testes. Fertil. Steril. 15:695-700, 1964

With O. Vilar, J.J. Hendrich, O.W. Davidson & B. Alvarez. Transference of circulating labeled serum proteins to the follicle of the rat ovary. J. Histochem. Cytochem. 11:80-8, 1963

With O. Vilar & M.I. Perez del Cerro. The Sertoli cells as a “bridge cell” between the basal membrane and the germinal cells. Histochemical and electron microscope observations. Exp. Cell Res. 237:158-61, 1962

With F.A. de la Balze, F. Arrillaga, M. Janches, O.W. Davidson & A.I. Gurtman. Male hypogonadism in hypothyroidism: a study of six cases. J. Clin. Endocrin. Metab. 22:212-22, 1962 

With R. Narbaitz & J.C. Lavieri. A histological and histochemical study of ten post-natal development of germinal epithelium and Sertoli cells. Anat. Rec. 133:410, 1959 

With M.F. Villamil. Tejido conectivo y enfermidades del colágeno (1959)

With D. Brandes. L’incorporation du soufre radioactif Si5 par les mucopolysaccharides de la crete du coq. C.R. Soc. Biol. Paris 150:2020-1, 1956  

With F.A. de la Balze, G.E. Bur & J. Irazu. Morphological and histochemical changes produced by estrogens on adult human testes. Fertil. Steril. 5:421-36, 1954

With J. Nolazco & F.A. de la Balze. Histochemical study of normal adult testes. Anat. Record 114:127, 1952

With M.H. Burgos. Mucopolisacaridios en la espermatogenesis del sapo. Rev. Soc. Arg. Biol. 24:318-27, 1948

Histochemical study of glycogen in tissues (1947)

With C. Bergada. Effect of gonadotropins on the induction of spermatogenesis in human prepubertal testis