João Consani Perrone, Brazilian biochemist (Passa Quatro, Minas Gerais 22 January 1922 – Rio de Janeiro 20 May 1979)

With colleagues the studies about castor bean cake gave them a number of scientific discoveries 

METHODS

Perrone method (chromatographic technique for separation of dinitrophenyl aminoacids) 

Separation of aminoacids as dinitrophenyl derivatives. Nature 167:513-5, 1951

A new method for chromatographic operating of peptides

Um novo método para o funcionamento cromatográfico de peptídeos. Rev. Quim. Ind. 26(300):16, 1957

With A. Iachan & R. Roitman. A method for the determination of proline and hydroxyproline. An. Acad. Bras. Ciencias 31(1):53-55, 1959

With A. Iachan & L.V. Disitzer. A new method for protein hydrolysis with crystallized oxalic acid. An. Acad. Bras. Ciencias 33(2):169-72, 1961

With G.B. Domont & A. Iachan developed 2 methods for the automatic determination of trypsin activity (1963)

Developed an industrial process for detoxifying castor bean cake

With A. Iachan, G.B. Domont, L.V. Disitzer, V.R.O. Castro, R. Roitman & S.M. Gomes. Contribuição ao estudo da torta de mamona (1966)

With R.A. Moreira. Purification and partial characterization of a lectin from Phaseolus vulgaris L. Plant Physiol. 59:783-7, 1977

SOME BIBLIOGRAPHY

With S. Rosenberg & P.L. Kirk. Amperometric titration of sulphydryl groups. Microgram Analysis. Anal. Chem. 22(9):1186-7, 1950

With W. Hull & P.L. Kirk. Tissue culture studies. V. Analogue for nicotinic acid. Journal Gen. Physiol. 34:75-9, 1950

With A. Neuberger & H.G.B. Slack. The relative metabolic inertia of tendon collagen in the rat. Biochem. J. 49(2):199-204, 1951

With H.M. Muir & A. Neuberger. Further isotopic studies on haemoglobin formation in the rat and rabbit. Biochem. J. 52(1):87-95, 1952

With L.D. Antonaccio. The salting-out curve of human serum. An. Acad. Bras. Ciencias 25(4):459-68, 1953

With A. Iachan & L.A. Moreira Carneiro. Reaction of formaldehyde with aromatic amino acids. I. Reaction with tryptophane. An. Acad. Bras. Ciencias 25:107-28, 1953

Reação do formaldeído com os acidos aminados básicos. II. Reação com a histidina. An. Acad. Bras. Ciencias 27(1):35-4, 1955

Cromatography of peptides in columns of cellulose powder. A preliminary note. An. Acad. Bras. Ciencias 28(4):501-8, 1956

With A. Iachan. Separação cromatográfica de ácidos aminados neutros em colunas de cellulose. An. Acad. Bras. Ciencias 30(2):17, 1958

With A. Iachan & L.V. Disitzer. Electrophoretic heterogeneity of trypsin. Nature 184:1225-6, 1959

With A. Iachan, L.V. Disitzer & G. B. Domont. Fractionation of trypsin by paper electrophoresis. Nature 203(4090):43-5, 1964

With L.V. Disitzer & G.B. Domont. The automatic determination of trypsin activity. An. Acad. Bras. Ciencias 36(2):131-5, 1964

Fracionamento eletroforético das proteínas da torta de mamona (1964)

Curvas de solubilidade das proteínas da torta da mamona (1964)

Destoxicação e desalergenização da torta da mamona (1964) 

With S.H. Kling, E. Solewicz, M.S.P. Daumas et al. Aproveitamento industrial das folhas de mandioca. Inform. Inst. Nac. Tecnol. 9:13-18, 1976   

Os processos hidrolíticos no aproveitamento dos recursos renováveis. Silvicultura 3:23-33, 1977

With D. Parreira & E. Rodrigues. Separação cromatográfica de ácidos aminados em colunas de “Dowex 50” pelo uso de um gradiente de pH. An. Ass. Bras. Quim. 15(1-4):29-32, 19??

With J. G. de Carvalho & V.R. Osorio e Castro. Reaction of glutaraldehyde with human erythrocytes. An. Acad. Bras. Ciencias 52(2):419-26, 1980